Authors

Mayas Gadid (1) , roula jacoub(2) , M. Issam Hasan Agha(3)

Abstract

An extraction of essential oil (EO) from Air Dried flowers of lavandula dentata L. Was carried out by hydro-distillation methods (Clevenger type), with yields (1.3ml /100 g dry weight), then (EO) was analyzed by GC and GC/MS. 15 components were detected, with 1,8-cineole (66.18) % as the major component. Antibacterial activity of (EO) and five pure standard materials (1.8-Cineol, Linalool, Camphor, β-pinene and α-pinene) was conducted at three concentrations (100%, 50% and 25%), against eight bacterial strains belong to four Gram-positive bacteria and three Gram-negative bacteria, According to disc diffusion method. Results showed that (EO) at concentration (100%) has exhibited inhibitory zones against all bacteria except Enterococcuse faecalis , In contrast only the studied Gram-negative bacteria (Salmonella typhimurium, Escherichia coli. and Pseudomonas aeruginosa) Were sensitive to the main component 1, 8 cineol at concentration (100%). Thus, present a rational for the synergistic effects of the diversity of major and minor Constituents in the essential oil of lavandula dentata L., which make the antibacterial activity of this oil higher, in comparison to that obtained by individual application of 1, 8 cineole as main component. Also, our results may motivate Local concerned to grow the French lavender under standards conditions to increase the accumulation of biosynthesized active constituents, and improve the oil’s composition, in order to realize a good bio-activity against human pathogens. 

Keywords

French lavender, lavandula dentata L., essential oil, GC-MS, antibacterial activity.