Micropropagation of Datura stramonium plant


Lama al-Ssawaf, Youssef  Al-Ammouri, SaleemZaid



This search was carried out in laboratory of Biotechnology of Medicinal and Aromatic Plants in National Commission for Biotechnology during 2012-2014, for studying effect of some growth regulators on length and number both of leaves and branches of Datura stramonium  plant. Seeds were surface disinfected by sodium hypochlorite and planted on free of growth regulators MS medium, where after, they were placed onto MS basal medium supported with 1mg\L IBA or NAA and (0.5, 1, 1.5, 2) mg\L BAP or K. Cultures were incubated in the growth room at 24 ±2 c and light intensity of 3000 luxat the cultures level. After that, plants were placed onto rooting media containing different concentration of IBA (0, 0.1, 0.5, 1, 2) mg\L.

Results showed that concentration 0.5  of sodium hypochlorite for 10 minutes was the best and gave 28% of germination per cent in average. While in multiplication media, control exceeded in length plant parameter in average 2,04 cm compression with all other treatments, but growth regulators increased the number both of branches and leaves, where MS3 and MS4 media exceeded in average of branches number ( 3.43-3.53 ) and (12.91، 14.64)for leaves number respectively. Higher root formation percent was 75% in MS medium supported with 2 mg\L IBA.


 Hyoscyamus aureus, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli

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