DETERMINATION OF TROPANE ALKALOIDS IN gOLDEN HENbANE (HyoscyAmus Aureus) iN viTro

  Authors

S. BESHER1, Y. AL-AMMOURI1, S. LAWAND2

 Abstract

 Aim. Aim of the work was to study tropane alkaloids in Hyoscyamus aureus plants methods. L-hyoscyamine and (α)-scopolamine were analyzed through GC-MS. results. Level of the tropane alkaloids (hyoscyamine and scopolamine) in plants under study late in the vegetative phase of development to be maintained in vitro was shown to depend
on ecotypes from which seeds were sampled. In general, hyoscyamine predominated in roots, while leaves demonstrated prevalence only one of the alkaloids with the other being in trace amounts. conclusions. Amount of alkaloids in roots was found to depend on altitude of plant vegetation as determined by its estimation in offspring generated in vitro. The idea that environment-genotype interaction may affect the amount of biologically active compounds produced by plant organism was confirmed.  

Keywords

> Hyoscyamus aureus, tropane alkaloids, culture maintained in vitro, GC-MS, L-hyoscyamine, (α)-scopolamine

 

 

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